3270 phospho p70 s6k (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc 3270 phospho p70 s6k
3270 Phospho P70 S6k, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 574 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phospho+c+jun+ser73/Phospho-c-Jun+(Ser73)+XP+Rabbit+mAb/pm41904147-229-116-113
Average 96 stars, based on 574 article reviews

3270 phospho p70 s6k - by Bioz Stars, 2026-10

96/100 stars

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Membrane:

Article Title: From Uremic Toxins to Hemodialysis Access Failure: IL-8 and MCP-1 Chemokines as a Link Between Endothelial Activation and AV Access Complications
Article Snippet: .. The membrane was incubated with primary antibodies directed against AHR (Santa Cruz Biotechnology, CliniSciences, Nanterre, France), phospho-p38 (Thr180/Tyr182), p38, phospho-c-Jun (Ser73), phospho-Smad2 (Ser465/Ser467), Smad2, β-actin, histone H3, or TATA box-binding protein (TBP) (all from Cell Signaling Technology, Ozyme, Saint-Cyr-l’Ecole, France), and then with secondary peroxidase-conjugated goat anti-mouse or anti-rabbit antibodies (Thermo Fisher Scientific, Illkirch, France). .. Revelation was made by chemiluminescence using ECL Western blotting substrate (Thermo Fisher Scientific, Illkirch, France).

Article Title: From Uremic Toxins to Hemodialysis Access Failure: IL-8 and MCP-1 Chemokines as a Link Between Endothelial Activation and AV Access Complications.
Article Snippet: .. The membrane was incubated with primary antibodies directed against AHR (Santa Cruz Biotechnology, CliniSciences, Nanterre, France), phospho-p38 (Thr180/Tyr182), p38, phospho-c-Jun (Ser73), phospho-Smad2 (Ser465/Ser467), Smad2, β-actin, histone H3, or TATA box-binding protein (TBP) (all from Cell Signaling Technology, Ozyme, Saint-Cyr-l’Ecole, France), and then with secondary peroxidase-conjugated goat anti-mouse or anti-rabbit antibodies (Thermo Fisher Scientific, Illkirch, France). .. Revelation was made by chemiluminescence using ECL Western blotting substrate (Thermo Fisher Scientific, Illkirch, France).

Incubation:

Article Title: DNA damage induced by HIV-1 Vpr triggers epigenetic remodeling and transcriptional programs to enhance virus transcription and latency reactivation
Article Snippet: Cells were blocked using PBST supplemented with 5% bovine serum albumin (BSA, Fisher Bioreagents BP9703100), 10% goat serum (Sigma-Aldrich, G9023-10mL), and 0.3 M glycine for 2 hours at room temperature while rocking. .. After blocking, samples were incubated with primary antibodies against γH2A.X (1:300, Cell Signaling 9718), pCHK1 (1:50, Cell Signaling 2348), pCHK2 (1:200, Cell Signaling 2661), acetyl-histone H2AK5 (1:1,000, Cell Signaling 2576), acetyl-histone H2A.Z K4/K7 (1:1,000, Cell Signaling 75336), total H2B (1:1,000, Cell Signaling 12364), acetyl-histone H2BK5 (1:1,000, Cell Signaling 12799), acetyl-histone H2BK12 (1:1,000, Cell Signaling 5410), acetyl-histone H2BK15 (1:1,000, Cell Signaling 9083), acetyl-histone H2BK20 (1:1,000, Cell Signaling 34156), total H3 (1:1,000, Cell Signaling 4499), phospho-histone H3S10 (1:1,000, Cell Signaling 53348), acetyl-histone H3K9 (1:1,000, Cell Signaling 9649), tri-methyl-histone H3K9 (1:1,000, Cell Signaling 13969), acetyl-histone H3K14 (1:1,000, Cell Signaling 7627), acetyl-histone H3K18 (1:1,000, Cell Signaling 13998), acetyl-histone H3K27 (1:1,000, Cell Signaling 8173T), tri-methyl histone H3K27 (1:1,000, Cell Signaling 9733), total H4 (1:1,000, Cell Signaling 2935), acetyl-histone H4K5 (1:1,000, Cell Signaling 8647), acetyl-histone H4K8 (1:1,000, Cell Signaling 2594), acetyl-histone H4K12 (1:1,000, Cell Signaling 13944T), Rpb1 CTD (1:1,000, Cell Signaling 2629T), Phospho-Rpb1 (Ser2) (1:1,000, Cell Signaling 13499T), Phospho-Rpb1 (Ser5) (1:1,000, Cell Signaling 13523T), DNA-PKcs (1:200, Cell Signaling 12311S), DNA-PKcs (phospho S2056) (1:200, Abcam ab18192), DCAF1/VprBP (1:400, Proteintech 11612-1-AP), NF-κB p65 (1:300, Cell Signaling 82425S), c-Jun (1:400, Cell Signaling 9165T), Phospho-c-Jun (Ser63) (1:1,000, Cell Signaling 91952T), Phospho-c-Jun (Ser73) (1:800, Cell Signaling 3270T), Sp1 (1:500, Cell Signaling 9389T), Phospho-Sp1 (Ser101) (1:300, Active Motif 39758), or S9.6 DNA-RNA hybrid (1:200, Kerafast ENH001) in blocking buffer overnight at 4 °C. .. The next day, cells were washed 3× with PBS in 5-min intervals and then incubated with anti-mCherry conjugated to Alexa Fluor 594 (1:800, Invitrogen M11240 ), secondary anti-rabbit-IgG conjugated to Alexa Fluor 488 (1:800, Cell Signaling 4412), secondary anti-rabbit-IgG conjugated to Alexa Fluor 594 (1:800, Cell Signaling 8889) or secondary anti-mouse-IgG conjugated to Alexa Fluor 488 (1:800, Cell Signaling 4408) in blocking buffer at room temperature for 1 hour.

Blocking Assay:

other:

Article Title: Targeted inhibition of M2 macrophages polarization via a PDC attenuates chronic pancreatitis through the PPARα pathway
Article Snippet: Phospho-c-Jun (Ser73) (D47G9) Rabbit Monoclonal Antibody , Cell Signaling Technology , Cat# 3270; RRID: AB_2129575.

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